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pet28 mhl expression vector  (Addgene inc)


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    Structured Review

    Addgene inc pet28 mhl expression vector
    Pet28 Mhl Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mhl+expression+vector/pET28-MHL+(Plasmid+%2326096)/pmc11954108__BLOODA_ADV___2024___014698___mmc1-24-21-24
    Average 94 stars, based on 39 article reviews
    pet28 mhl expression vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Direct inhibition of oncogenic KRAS by hydrocarbon-stapled SOS1 helices
    Article Snippet: .. Recombinant wild-type human KRAS protein (amino acids1–156) and its G12D, G12V, G12S, G12C, and Q61H mutant variants were expressed in Escherichia coli BL21(DE3) as N-terminal hexahistidine-tagged (His 6 ) fusion proteins by using the pET28-MHL expression vector (Addgene plasmid 25153; a gift from C. Arrowsmith, University of Toronto, Toronto). ..

    Mutagenesis:

    Article Title: Direct inhibition of oncogenic KRAS by hydrocarbon-stapled SOS1 helices
    Article Snippet: .. Recombinant wild-type human KRAS protein (amino acids1–156) and its G12D, G12V, G12S, G12C, and Q61H mutant variants were expressed in Escherichia coli BL21(DE3) as N-terminal hexahistidine-tagged (His 6 ) fusion proteins by using the pET28-MHL expression vector (Addgene plasmid 25153; a gift from C. Arrowsmith, University of Toronto, Toronto). ..

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.

    Expressing:

    Article Title: Direct inhibition of oncogenic KRAS by hydrocarbon-stapled SOS1 helices
    Article Snippet: .. Recombinant wild-type human KRAS protein (amino acids1–156) and its G12D, G12V, G12S, G12C, and Q61H mutant variants were expressed in Escherichia coli BL21(DE3) as N-terminal hexahistidine-tagged (His 6 ) fusion proteins by using the pET28-MHL expression vector (Addgene plasmid 25153; a gift from C. Arrowsmith, University of Toronto, Toronto). ..

    Article Title: Germline loss of MBD4 predisposes to leukaemia due to a mutagenic cascade driven by 5mC
    Article Snippet: PCR products were purified using a Millipore MicroscreenTM PCR cleanup plate (Merck, Kenilworth, NJ, USA) and sequenced on an Applied Biosystems 3130xl Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). .. Full length DNMT3A and MBD4 cDNA were PCR amplified from a pReceiver-B13 vector (GeneCopoeia, Rockville MD, USA) and cloned into pET28-MHL expression vector (GenBank accession EF456735 , Addgene, Cambridge, MA, USA) by applying BD-BioScience In-Fusion (Franklin Lakes, NJ, USA) enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products, using the following primers for DNMT3A: sense 5’-TTGTATTTCCAGGGCCCCGCCATGCCCTCCAGC-3’ and anti-sense 5’-CAAGCTTCGTCATCACACACACGCAAAATACTCCTTCAGC-3’, MBD4: sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT and anti-sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT-3’. .. The truncated form of MBD4, residues 430-580, in the pET28 backbone was sourced from Addgene.

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.

    Plasmid Preparation:

    Article Title: Direct inhibition of oncogenic KRAS by hydrocarbon-stapled SOS1 helices
    Article Snippet: .. Recombinant wild-type human KRAS protein (amino acids1–156) and its G12D, G12V, G12S, G12C, and Q61H mutant variants were expressed in Escherichia coli BL21(DE3) as N-terminal hexahistidine-tagged (His 6 ) fusion proteins by using the pET28-MHL expression vector (Addgene plasmid 25153; a gift from C. Arrowsmith, University of Toronto, Toronto). ..

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.

    Polymerase Chain Reaction:

    Article Title: Germline loss of MBD4 predisposes to leukaemia due to a mutagenic cascade driven by 5mC
    Article Snippet: PCR products were purified using a Millipore MicroscreenTM PCR cleanup plate (Merck, Kenilworth, NJ, USA) and sequenced on an Applied Biosystems 3130xl Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). .. Full length DNMT3A and MBD4 cDNA were PCR amplified from a pReceiver-B13 vector (GeneCopoeia, Rockville MD, USA) and cloned into pET28-MHL expression vector (GenBank accession EF456735 , Addgene, Cambridge, MA, USA) by applying BD-BioScience In-Fusion (Franklin Lakes, NJ, USA) enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products, using the following primers for DNMT3A: sense 5’-TTGTATTTCCAGGGCCCCGCCATGCCCTCCAGC-3’ and anti-sense 5’-CAAGCTTCGTCATCACACACACGCAAAATACTCCTTCAGC-3’, MBD4: sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT and anti-sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT-3’. .. The truncated form of MBD4, residues 430-580, in the pET28 backbone was sourced from Addgene.

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.

    Amplification:

    Article Title: Germline loss of MBD4 predisposes to leukaemia due to a mutagenic cascade driven by 5mC
    Article Snippet: PCR products were purified using a Millipore MicroscreenTM PCR cleanup plate (Merck, Kenilworth, NJ, USA) and sequenced on an Applied Biosystems 3130xl Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). .. Full length DNMT3A and MBD4 cDNA were PCR amplified from a pReceiver-B13 vector (GeneCopoeia, Rockville MD, USA) and cloned into pET28-MHL expression vector (GenBank accession EF456735 , Addgene, Cambridge, MA, USA) by applying BD-BioScience In-Fusion (Franklin Lakes, NJ, USA) enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products, using the following primers for DNMT3A: sense 5’-TTGTATTTCCAGGGCCCCGCCATGCCCTCCAGC-3’ and anti-sense 5’-CAAGCTTCGTCATCACACACACGCAAAATACTCCTTCAGC-3’, MBD4: sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT and anti-sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT-3’. .. The truncated form of MBD4, residues 430-580, in the pET28 backbone was sourced from Addgene.

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.

    Clone Assay:

    Article Title: Germline loss of MBD4 predisposes to leukaemia due to a mutagenic cascade driven by 5mC
    Article Snippet: PCR products were purified using a Millipore MicroscreenTM PCR cleanup plate (Merck, Kenilworth, NJ, USA) and sequenced on an Applied Biosystems 3130xl Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). .. Full length DNMT3A and MBD4 cDNA were PCR amplified from a pReceiver-B13 vector (GeneCopoeia, Rockville MD, USA) and cloned into pET28-MHL expression vector (GenBank accession EF456735 , Addgene, Cambridge, MA, USA) by applying BD-BioScience In-Fusion (Franklin Lakes, NJ, USA) enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products, using the following primers for DNMT3A: sense 5’-TTGTATTTCCAGGGCCCCGCCATGCCCTCCAGC-3’ and anti-sense 5’-CAAGCTTCGTCATCACACACACGCAAAATACTCCTTCAGC-3’, MBD4: sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT and anti-sense 5’-TTGTATTTCCAGGGCGGCACGACTGGGCTGGAGAGTCT-3’. .. The truncated form of MBD4, residues 430-580, in the pET28 backbone was sourced from Addgene.

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.

    Cloning:

    Article Title: Double mutant DNMT3A AML: a unique subtype experiencing increased DNA damage and poor prognosis
    Article Snippet: .. Cloning and site-directed mutagenesis of DNMT3A Full length DNMT3A cDNA was PCR amplified from a pReceiver-B13 vector (Genecopoeia) and cloned into pET28-MHL expression vector (Addgene, GenBank accession EF456735) by applying BD-BioScience Infusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at the end of the PCR products. .. A QuickChange II XL site-directed mutagenesis kit (Stratagene) was used to generate the various DNMT3A mutants.



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